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Each capsule delivers 250mg of L-Theanine from green tea leaf (Camellia sinensis) a naturally occurring non-protein amino acid found almost exclusively in the tea plant. L-Theanine is the compound responsible for the calm, focused quality of green tea that distinguishes it from other caffeinated beverages. At 250mg per capsule, this formula delivers the L-Theanine equivalent of approximately 5–12 cups of brewed green tea without caffeine. 

What Is L-Theanine?

L-Theanine (γ-glutamylethylamide) is a non protein amino acid found almost exclusively in the leaves of Camellia sinensis the tea plant from which green tea, black tea, white tea, and oolong tea are derived. It is one of the primary bioactive compounds in green tea alongside caffeine and catechin polyphenols. A standard cup of green tea contains approximately 20–50mg of L-Theanine depending on variety, growing conditions, and brewing method. L-Theanine crosses the blood-brain barrier efficiently after oral ingestion, reaching peak plasma concentrations within approximately 30–60 minutes.

The Alpha Wave Mechanism: Relaxed Alertness Without Drowsiness

The most consistently documented neurological effect of L-Theanine is its ability to increase alpha brain wave activity (8–13 Hz) the wave pattern associated with relaxed, wakeful alertness: calm but mentally present, without tension or drowsiness. Multiple EEG studies have demonstrated that L-Theanine increases alpha wave activity in the occipital and parietal regions within 30–60 minutes of ingestion. Most relaxation compounds produce sedation alongside relaxation by broadly increasing inhibitory neurotransmission. L-Theanine's alpha wave promotion produces a qualitatively different state calm without drowsiness appropriate for daytime use, work, and study.

Neurochemical Mechanisms

Glutamate receptor modulation: Structurally similar to glutamate, L-Theanine acts as a partial agonist at NMDA receptors and inhibits glutamate uptake at AMPA and kainate receptors, moderating excitatory neurotransmission without fully blocking it. GABA support: L-Theanine increases GABA (gamma-aminobutyric acid) levels, the primary inhibitory neurotransmitter, contributing to calm and relaxation. Dopamine modulation: L-Theanine increases dopamine in certain brain regions, supporting motivation, mood, and focused mental state under stress. Serotonin: L-Theanine may also influence serotonin levels, contributing to mood balance and emotional resilience.

L-Theanine 250mg | Relaxation Without Drowsiness | Stress Support | 300 Vcaps

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Health Canada Licensed Natural Health Product (NPN 80068090)

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Each capsule delivers 250mg of L-Theanine from green tea leaf (Camellia sinensis) a naturally occurring non-protein amino acid found almost exclusively in the tea plant. L-Theanine is the compound responsible for the calm, focused quality of green tea that distinguishes it from other caffeinated beverages. At 250mg per capsule, this formula delivers the L-Theanine equivalent of approximately 5–12 cups of brewed green tea without caffeine. 

What Is L-Theanine?

L-Theanine (γ-glutamylethylamide) is a non protein amino acid found almost exclusively in the leaves of Camellia sinensis the tea plant from which green tea, black tea, white tea, and oolong tea are derived. It is one of the primary bioactive compounds in green tea alongside caffeine and catechin polyphenols. A standard cup of green tea contains approximately 20–50mg of L-Theanine depending on variety, growing conditions, and brewing method. L-Theanine crosses the blood-brain barrier efficiently after oral ingestion, reaching peak plasma concentrations within approximately 30–60 minutes.

The Alpha Wave Mechanism: Relaxed Alertness Without Drowsiness

The most consistently documented neurological effect of L-Theanine is its ability to increase alpha brain wave activity (8–13 Hz) the wave pattern associated with relaxed, wakeful alertness: calm but mentally present, without tension or drowsiness. Multiple EEG studies have demonstrated that L-Theanine increases alpha wave activity in the occipital and parietal regions within 30–60 minutes of ingestion. Most relaxation compounds produce sedation alongside relaxation by broadly increasing inhibitory neurotransmission. L-Theanine's alpha wave promotion produces a qualitatively different state calm without drowsiness appropriate for daytime use, work, and study.

Neurochemical Mechanisms

Glutamate receptor modulation: Structurally similar to glutamate, L-Theanine acts as a partial agonist at NMDA receptors and inhibits glutamate uptake at AMPA and kainate receptors, moderating excitatory neurotransmission without fully blocking it. GABA support: L-Theanine increases GABA (gamma-aminobutyric acid) levels, the primary inhibitory neurotransmitter, contributing to calm and relaxation. Dopamine modulation: L-Theanine increases dopamine in certain brain regions, supporting motivation, mood, and focused mental state under stress. Serotonin: L-Theanine may also influence serotonin levels, contributing to mood balance and emotional resilience.

Supplement Facts

Supplement Facts

Serving Size: 1 Capsule

Servings Per Container: 300

Ingredient
Amount Per
Serving
% Daily
Value
L-Theanine (from Green Tea Leaf, Camellia sinensis)
250 mg

† Daily Value not established.

Other Ingredients:
Microcrystalline Cellulose, Hypromellose (capsule shell). Vegan. Non-GMO. Gluten Free. Dairy Free. Soy Free. Caffeine Free.

Clinical Research

L-Theanine, a Natural Constituent in Tea, and Its Effect on Mental State

Asia Pacific Journal of Clinical Nutrition, 2008

Randomized, placebo controlled study using EEG to measure the effects of L-Theanine on brain wave activity and mental state in healthy adults. Results demonstrated a significant increase in alpha brain wave activity (8–13 Hz) in the occipital and parietal regions within 30–60 minutes of L-Theanine ingestion compared to placebo. Alpha waves are associated with relaxed, wakeful alertness calm but mentally present, without drowsiness or sedation. Provides the neurophysiological basis for L-Theanine's ability to promote relaxation without drowsiness

L-Theanine Reduces Psychological and Physiological Stress Responses

Biological Psychology, 2007

Randomized, placebo controlled study examining the effects of L-Theanine on psychological and physiological stress responses in healthy adults. Results showed that L-Theanine significantly attenuated heart rate and salivary immunoglobulin A responses to an acute stress task compared to placebo, and reduced subjective stress scores.

The Combined Effects of L-Theanine and Caffeine on Cognitive Performance and Mood

Nutritional Neuroscience, 2008

Randomized, double blind, placebo controlled crossover study examining the effects of L-Theanine alone, caffeine alone, and their combination on cognitive performance and mood in healthy adults. L-Theanine alone improved speed and accuracy of attention-switching tasks and reduced susceptibility to distracting information compared to placebo. The study provides direct evidence for L-Theanine's independent cognitive performance-supporting effects

Research information is provided for educational purposes and does not replace the product's approved label information.

Frequently Asked Questions

What is L-Theanine and what does it do?

L-Theanine (γ-glutamylethylamide) is a naturally occurring non-protein amino acid found almost exclusively in the leaves ofCamellia sinensis the tea plant. It is the compound responsible for the calm, focused quality of green tea that distinguishes it from other caffeinated beverages. Licensed by Health Canada (NPN 80068090) to help temporarily promote relaxation without drowsiness, help reduce stress, and help support cognitive function. Each capsule provides 250mg of L-Theanine from green tea leaf equivalent to approximately 5–12 cups of brewed green tea, without caffeine.

How does L-Theanine promote relaxation without drowsiness?

L-Theanine's primary neurological mechanism is the promotion of alpha brain wave activity (8–13 Hz) the wave pattern associated with relaxed, wakeful alertness: calm but mentally present, without tension or sedation. Multiple EEG studies have demonstrated that L-Theanine increases alpha wave activity in the occipital and parietal regions within 30–60 minutes of ingestion. Most relaxation compounds produce sedation alongside relaxation by broadly increasing inhibitory neurotransmission. L-Theanine's alpha wave promotion produces a qualitatively different state calm without drowsiness appropriate for daytime use, work, and study.

What are the neurochemical mechanisms of L-Theanine?

L-Theanine influences brain chemistry through four complementary mechanisms. First, glutamate receptor modulation: structurally similar to glutamate, L-Theanine acts as a partial agonist at NMDA receptors and moderates excitatory neurotransmission without fully blocking it. Second, GABA support: L-Theanine increases GABA (gamma-aminobutyric acid) levels, the primary inhibitory neurotransmitter, contributing to calm and relaxation. Third, dopamine modulation: L-Theanine increases dopamine in certain brain regions, supporting motivation, mood, and focused mental state under stress. Fourth, serotonin influence: L-Theanine may also support serotonin levels, contributing to mood balance and emotional resilience.

Why is 250mg considered a high potency dose of L-Theanine?

A standard cup of green tea contains approximately 20–50mg of L-Theanine depending on variety, growing conditions, and brewing method. At 250mg per capsule, one capsule provides the L-Theanine equivalent of approximately 5–12 cups of brewed green tea without caffeine, tannins, or liquid volume. Most L-Theanine supplements on the market contain 100–200mg per capsule. The 250mg dose is at the upper end of commonly available supplemental doses and is consistent with the higher doses used in clinical research on L-Theanine's stress and cognitive effects.

Who should not take L-Theanine 250mg?

Consult a healthcare practitioner before use if you are pregnant or breastfeeding, or if you are taking medications for blood pressure or stimulant medications. L-Theanine may have mild blood pressure lowering effects; individuals taking antihypertensive medications should consult a healthcare provider before use.